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Sci. Signal., 27 September 2011
[DOI: 10.1126/scisignal.2002049]

Supplementary Materials for:

Tyrosine Phosphorylation of the Gα-Interacting Protein GIV Promotes Activation of Phosphoinositide 3-Kinase During Cell Migration

Changsheng Lin, Jason Ear, Yelena Pavlova, Yash Mittal, Irina Kufareva, Majid Ghassemian, Ruben Abagyan, Mikel Garcia-Marcos, Pradipta Ghosh*

*To whom correspondence should be addressed. E-mail: prghosh{at}

This PDF file includes:

  • Fig. S1. GIV is phosphorylated in vitro by receptor tyrosine kinases and in cells in response to growth factors.
  • Fig. S2. Bioinformatic analyses of the phosphopeptide sequences of GIV.
  • Fig. S3. Both EGFR and Src can phosphorylate GIV after EGF stimulation.
  • Fig. S4. The GEF domain is not required for tyrosine phosphorylation of GIV.
  • Fig. S5. GIV interacts with p85α and EGFR upon growth factor stimulation.
  • Fig. S6. Molecular modeling of the interfaces between the SH2 domains of p85α and the GIV-derived phosphopeptides.
  • Fig. S7. PIP3 production and localization as detected by a Btk-based reporter.
  • pTyrGIVCT file description
  • References

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Technical Details

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Size: 1.22 MB

Other Supplementary Material for this manuscript includes the following:

  • pTyrGIVCT files

[Download pTyrGIVCT files (Compressed)]

Technical Details

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Size: 6.62 MB (compressed); 12.8 MB (decompressed)

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Citation: C. Lin, J. Ear, Y. Pavlova, Y. Mittal, I. Kufareva, M. Ghassemian, R. Abagyan, M. Garcia-Marcos, P. Ghosh, Tyrosine Phosphorylation of the Gα-Interacting Protein GIV Promotes Activation of Phosphoinositide 3-Kinase During Cell Migration. Sci. Signal. 4, ra64 (2011).

© 2011 American Association for the Advancement of Science

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