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Sci. Signal., 4 October 2011
[DOI: 10.1126/scisignal.2001821]

Supplementary Materials for:

Itk Controls the Spatiotemporal Organization of T Cell Activation

Kentner L. Singleton, Monica Gosh, Radhika D. Dandekar, Byron B. Au-Yeung, Olga Ksionda, Victor L. J. Tybulewicz, Amnon Altman, Deborah J. Fowell, Christoph Wülfing*

*To whom correspondence should be addressed. E-mail: christoph.wuelfing{at}UTSouthwestern.edu

This PDF file includes:

  • Fig. S1. Itk is part of the cSMAC signaling complex.
  • Fig. S2. Itk regulates multiple elements of the spatiotemporal organization of T cell signaling.
  • Fig. S3. Centrally targeted active Cdc42 does not restore actin patterning, TCR patterning, or IL-2 secretion in Itk-deficient T cells.
  • Fig. S4. Itk regulates the recruitment of Cdc42 GEFs to the T cell–APC interface.
  • Fig. S5. SLAT knockdown results in a phenotype that resembles that of Itk deficiency.
  • Fig. S6. TCR clustering is diminished in TH2 cells and the Itk SH2 domain is required for efficient recruitment of Itk to the cSMAC.
  • Table S1. Movie descriptions.

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.26 MB

Other Supplementary Material for this manuscript includes the following:

  • Movies S1 to S18 (.mov format)

[Download Movies S1 to S18 (Compressed)]

Technical Details

Format: Quicktime Movie. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 5.7 MB (compressed); 16.2 MB (decompressed)


Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: K. L. Singleton, M. Gosh, R. D. Dandekar, B. B. Au-Yeung, O. Ksionda, V. L. J. Tybulewicz, A. Altman, D. J. Fowell, C. Wülfing, Itk Controls the Spatiotemporal Organization of T Cell Activation. Sci. Signal. 4, ra66 (2011).

© 2011 American Association for the Advancement of Science


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