Itk Controls the Spatiotemporal Organization of T Cell Activation

Kentner L. Singleton, Monica Gosh, Radhika D. Dandekar, Byron B. Au-Yeung, Olga Ksionda, Victor L. J. Tybulewicz, Amnon Altman, Deborah J. Fowell, Christoph Wülfing*

*To whom correspondence should be addressed. E-mail: christoph.wuelfing{at}UTSouthwestern.edu

This PDF file includes:

• Fig. S1. Itk is part of the cSMAC signaling complex.
• Fig. S2. Itk regulates multiple elements of the spatiotemporal organization of T cell signaling.
• Fig. S3. Centrally targeted active Cdc42 does not restore actin patterning, TCR patterning, or IL-2 secretion in Itk-deficient T cells.
• Fig. S4. Itk regulates the recruitment of Cdc42 GEFs to the T cell–APC interface.
• Fig. S5. SLAT knockdown results in a phenotype that resembles that of Itk deficiency.
• Fig. S6. TCR clustering is diminished in T_H2 cells and the Itk SH2 domain is required for efficient recruitment of Itk to the cSMAC.
• Table S1. Movie descriptions.

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© 2011 American Association for the Advancement of Science