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Sci. Signal., 8 November 2011
[DOI: 10.1126/scisignal.2001993]

Supplementary Materials for:

Systematic Phosphorylation Analysis of Human Mitotic Protein Complexes

Björn Hegemann, James R. A. Hutchins, Otto Hudecz, Maria Novatchkova, Jonathan Rameseder, Martina M. Sykora, Sihan Liu, Michael Mazanek, Péter Lénárt, Jean-Karim Hériché, Ina Poser, Norbert Kraut, Anthony A. Hyman, Michael B. Yaffe, Karl Mechtler, Jan-Michael Peters*

*To whom correspondence should be addressed. E-mail: peters{at}imp.ac.at

This PDF file includes:

  • Fig. S1. Visualization of phosphosites within the human mitotic interaction network with Cytoscape.
  • Fig. S2. Statistical analysis of the frequency of occurrence of kinase motifs matching phosphosites in the mitotic interaction data set.
  • Fig. S3. BI 4834 is structurally and functionally related to BI 2536.
  • Fig. S4. Manual evaluation of phosphopeptide multistage activation (MSA) spectra.
  • Fig. S5. Further analysis with phosphospecific antibodies..

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.37 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of all detected phosphorylation sites in the interaction data set, including Scansite matches and Ascores.
  • Table S2 (Microsoft Excel format). List of sequence coverages for the proteins analyzed in the interaction data set, including phosphorylation site densities in percent.
  • Table S3 (Microsoft Excel format). List of all detected phosphorylation sites in the four-condition data set, including Scansite matches.
  • Table S4 (Microsoft Excel format). List of sequence coverages for the proteins analyzed in the four-condition set, including phosphorylation site densities in percent.
  • Table S5 (Microsoft Excel format). Access to experimental and RAW file data.
  • Cytoscape file S1 (.cys format). Interaction data set, including information on cluster membership, number of phosphorylation sites, phosphorylation site density in percent, and protein sequence coverage.

[Download Tables S1 to S5 (Compressed)]

Technical Details

Format: Microsoft Excel. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 679 KB (compressed); 2 MB (decompressed)

[Download Cytoscape file S1 (Compressed)]

Technical Details

Format: Cytoscape. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 85 KB (compressed); 87 KB (decompressed)

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; www.winzip.com) or StuffIt Expander (Windows and Mac; www.stuffit.com).
Citation: B. Hegemann, J. R. A. Hutchins, O. Hudecz, M. Novatchkova, J. Rameseder, M. M. Sykora, S. Liu, M. Mazanek, P. Lénárt, J.-K. Hériché, I. Poser, N. Kraut, A. A. Hyman, M. B. Yaffe, K. Mechtler, J.-M. Peters, Systematic Phosphorylation Analysis of Human Mitotic Protein Complexes. Sci. Signal. 4, rs12 (2011).

© 2011 American Association for the Advancement of Science

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