Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca²⁺/Calmodulin–Dependent Protein Kinases

Shinichi Nakamuta, Yasuhiro Funahashi, Takashi Namba, Nariko Arimura, Marina R. Picciotto, Hiroshi Tokumitsu, Thomas R. Soderling, Akira Sakakibara, Takaki Miyata, Hiroyuki Kamiguchi, Kozo Kaibuchi*

*To whom correspondence should be addressed. E-mail: kaibuchi{at}med.nagoya-u.ac.jp

This PDF file includes:

• Materials and Methods
• Fig. S1. TrkB and TrkC signaling contributes to neuronal polarization.
• Fig. S2. NT-3–stimulated neurite becomes an axon.
• Fig. S3. Local application of BDNF induces neurite outgrowth.
• Fig. S4. CaMKK and CaMKI inhibitors prevent neuronal polarization.
• Fig. S5. CaMKKα-CA induces multiple axons.
• Fig. S6. CaMKIα contributes to neuronal polarization.
• Fig. S7. CaMKKα is expressed in multipolar neurons.
• Fig. S8. Migration and morphology of cortical neurons.
• Fig. S9. Morphological analysis of EGFP+ multipolar cells.
• Fig. S10. CaMKKα contributes to neuronal polarization in vivo.
• Fig. S11. Expression of CaMKKα-DN under the control of the CAG promoter impairs neuronal migration in vivo.
• Fig. S12. Expression of TrkB, TrkC, or CaMKKα impairs neuronal polarization and neuronal migration in vivo at E18.

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Multipolar-to-bipolar transition of neurons in the embryonic neocortex.
• Movie S2 (.mp4 format). Migration and morphology of control cortical neurons.
• Movie S3 (.mp4 format). Migration and morphology of cortical neurons electroporated with wild-type CaMKKa.
• Movie S4 (.mp4 format). Migration and morphology of cortical neurons electroporated with CaMKKα-CA.
• Movie S5 (.mp4 format). Migration and morphology of cortical neurons electroporated with CaMKKα-DN.

© 2011 American Association for the Advancement of Science