SH3 Domain–Based Phototrapping in Living Cells Reveals Rho Family GAP Signaling Complexes

Hirokazu Okada, Akiyoshi Uezu, Frank M. Mason, Erik J. Soderblom, M. Arthur Moseley III, Scott H. Soderling*

*To whom correspondence should be addressed. E-mail: s.soderling{at}cellbio.duke.edu

This PDF file includes:

• Materials and Methods
• Fig. S1. Expression levels of Rho family GAP SH3 domains and their interactors.
• Fig. S2. Identification of SH3 domain binding proteins by peptide array–based in vitro binding assay and coimmunoprecipitation.
• Fig. S3. Rho family GAP PI cluster interactomes and colocalization data.
• Fig. S4. Gephyrin's interaction with WRP and colocalization with VIAAT.
• Fig. S5. Endogenous gephyrin is highly colocalized with inhibitory synapse presynaptic marker VIAAT in vivo.
• Reference

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© 2011 American Association for the Advancement of Science