Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 6 December 2011
[DOI: 10.1126/scisignal.2002105]

Supplementary Materials for:

The SH2 Domain–Containing Proteins in 21 Species Establish the Provenance and Scope of Phosphotyrosine Signaling in Eukaryotes

Bernard A. Liu, Eshana Shah, Karl Jablonowski, Andrew Stergachis, Brett Engelmann, Piers D. Nash*

*To whom correspondence should be addressed. E-mail: pdnash.uchicago{at}

This PDF file includes:

  • Section S1. SH2 domain proteins in organisms with incomplete genomes.
  • Section S2. Analysis of the intron/exon code of human SH2 domains.
  • Section S3. Comparative analysis of ortholog and paralog predictions.
  • Fig. S1. An evolutionary time line for the organisms represented in this study.
  • Fig. S2. Evolutionary expansion of SH2 domains, tyrosine kinases, and PDZ domains.
  • Fig. S3. Splice site positions within the protein sequence alignment of human SH2 domains.
  • Fig. S4. The bead on a string representation for protein domains.
  • Fig. S5. Gene duplication and loss within SH2 domain families.
  • Fig. S6. Clustal alignments of the GRB2 and CRK families.
  • Fig. S7. Insertion of an intramolecular phosphorylation site for autoinhibition of CRK.
  • Fig. S8. Evolving new SH2 interactions through novel pTyr sites.
  • Fig. S9. Conservation of the pTyr ligand-binding pocket in SH2 domains.
  • Fig. S10. Tissue expression of human SH2 domain proteins.
  • Table S1. A complete list of organisms in this study.
  • Table S2. SH2 domains in Bikonta and Amoebozoa.
  • Table S5. Classification of SH2 domain family divergence.
  • Table S6. Defining SH2 families with Ensembl paralog predictions versus family organization.
  • Table S7. Ortholog predictions of human SH2 proteins to proteins in lower organisms.
  • Table S8. Ortholog predictions from C. elegans to H. sapiens.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 11 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S3. Comprehensive list of SH2 domain–containing proteins in Eukaryotes (Excel file).
  • Table S4. Comprehensive list of SH2 domain–containing proteins in organisms with incomplete genome annotations (Excel file).

[Download Tables S3. and S4. (compressed)]

Technical Details

Format: Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 176 KB (compressed); 542 KB (decompressed)

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; or StuffIt Expander (Windows and Mac;

Citation: B. A. Liu, E. Shah, K. Jablonowski, A. Stergachis, B. Engelmann, P. D. Nash, The SH2 Domain–Containing Proteins in 21 Species Establish the Provenance and Scope of Phosphotyrosine Signaling in Eukaryotes. Sci. Signal. 4, ra83 (2011).

© 2011 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882