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Sci. Signal., 20 December 2011
[DOI: 10.1126/scisignal.2002179]

Supplementary Materials for:

Human Regulatory T Cells Rapidly Suppress T Cell Receptor–Induced Ca2+, NF-κB, and NFAT Signaling in Conventional T Cells

Angelika Schmidt, Nina Oberle, Eva-Maria Weiß, Diana Vobis, Stefan Frischbutter, Ria Baumgrass, Christine S. Falk, Mathias Haag, Britta Brügger, Hongying Lin, Georg W. Mayr, Peter Reichardt, Matthias Gunzer, Elisabeth Suri-Payer, Peter H. Krammer*

*To whom correspondence should be addressed. E-mail: P.Krammer{at}dkfz.de

This PDF file includes:

  • Methods
  • Fig. S1. Suppression of cytokine secretion is retained after removal of Tregs.
  • Fig. S2. Treg-mediated rapid suppression of IL2 expression in Tcons also occurs in the presence of APCs.
  • Fig. S3. CTLA-4 is not involved in the suppression of cytokine gene expression.
  • Fig. S4. Proximal TCR signaling is not altered in suppressed Tcons.
  • Fig. S5. Suppression of NFAT and NF-κB activation is independent of the antibody used to activate Tregs.
  • Fig. S6. Phosphorylation of ERK and p38 is not affected in suppressed Tcons.
  • Fig. S7. The block in Ca2+ influx in Tcons is not caused by allogeneic responses and requires 30 min of previous coculture with Tregs.
  • Fig. S8. Suppression of Ca2+ signaling is independent of cAMP.
  • Fig. S9. Tregs, but not Tcons, pre-activated with covalently plate-bound antibody against CD3 suppress Ca2+ signaling in responder Tcons.
  • Fig. S10. NFAT and NF-κB activation in Tcons is Ca2+-dependent.
  • References

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.07 MB


Citation: A. Schmidt, N. Oberle, E.-M. Weiß, D. Vobis, S. Frischbutter, R. Baumgrass, C. S. Falk, M. Haag, B. Brügger, H. Lin, G. W. Mayr, P. Reichardt, M. Gunzer, E. Suri-Payer, P.H. Krammer, Human Regulatory T Cells Rapidly Suppress T Cell Receptor–Induced Ca2+, NF-κB, and NFAT Signaling in Conventional T Cells. Sci. Signal. 4, ra90 (2011).

© 2011 American Association for the Advancement of Science


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