Extracellular Ca²⁺ Acts as a Mediator of Communication from Neurons to Glia

Arnulfo Torres, Fushun Wang, Qiwu Xu, Takumi Fujita, Radoslaw Dobrowolski, Klaus Willecke, Takahiro Takano, Maiken Nedergaard*

*To whom correspondence should be addressed. E-mail: nedergaard{at}urmc.rochester.edu

This PDF file includes:

• Fig. S1. Calibration of Ca²⁺-sensitive microelectrode and effects of diazo-2 photolysis on [Ca²⁺]_e.
• Fig. S2. CA1 pyramidal neurons depolarize and decrease their input resistance in response to photolysis of MNI-glutamate, whereas photolysis of diazo-2 is not associated with changes in the membrane properties.
• Fig. S3. Photolysis of MNI-glutamate.
• Fig. S4. Transgenic reporter mice reveal distinct responses to MNI-glutamate photolysis of neurons and astrocytes in hippocampal slices.
• Fig. S5. Comparison of Ca²⁺ waves in hippocampus and cortex evoked by photolysis of MNI-glutamate and diazo-2.
• Fig. S6. Comparison of Ca²⁺ waves evoked by diazo-2 and MNI-glutamate photolysis.
• Fig. S7. Effect of manipulation of P2Y1 receptors in hippocampal interneurons.
  

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© 2012 American Association for the Advancement of Science