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Sci. Signal., 24 January 2012
[DOI: 10.1126/scisignal.2002160]

Supplementary Materials for:

Extracellular Ca2+ Acts as a Mediator of Communication from Neurons to Glia

Arnulfo Torres, Fushun Wang, Qiwu Xu, Takumi Fujita, Radoslaw Dobrowolski, Klaus Willecke, Takahiro Takano, Maiken Nedergaard*

*To whom correspondence should be addressed. E-mail: nedergaard{at}

This PDF file includes:

  • Fig. S1. Calibration of Ca2+-sensitive microelectrode and effects of diazo-2 photolysis on [Ca2+]e.
  • Fig. S2. CA1 pyramidal neurons depolarize and decrease their input resistance in response to photolysis of MNI-glutamate, whereas photolysis of diazo-2 is not associated with changes in the membrane properties.
  • Fig. S3. Photolysis of MNI-glutamate.
  • Fig. S4. Transgenic reporter mice reveal distinct responses to MNI-glutamate photolysis of neurons and astrocytes in hippocampal slices.
  • Fig. S5. Comparison of Ca2+ waves in hippocampus and cortex evoked by photolysis of MNI-glutamate and diazo-2.
  • Fig. S6. Comparison of Ca2+ waves evoked by diazo-2 and MNI-glutamate photolysis.
  • Fig. S7. Effect of manipulation of P2Y1 receptors in hippocampal interneurons.

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Citation: A. Torres, F. Wang, Q. Xu, T. Fujita, R. Dobrowolski, K. Willecke, T. Takano, M. Nedergaard, Extracellular Ca2+ Acts as a Mediator of Communication from Neurons to Glia. Sci. Signal. 5, ra8 (2012).

© 2012 American Association for the Advancement of Science

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