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Sci. Signal., 31 January 2012
[DOI: 10.1126/scisignal.2002446]

Supplementary Materials for:

Myosin I Links PIP3 Signaling to Remodeling of the Actin Cytoskeleton in Chemotaxis

Chun-Lin Chen, Yu Wang, Hiromi Sesaki, Miho Iijima*

*To whom correspondence should be addressed. E-mail: miijima{at}jhmi.edu

This PDF file includes:

  • Fig. S1. Immunopurified myosin IE–GFP binds to PIP3.
  • Fig. S2. Liposome binding assays.
  • Fig. S3. Quantification of myosin IE–GFP localization.
  • Fig. S4. Generation of myosin I–null cells.
  • Fig. S5. Myosin IE–GFP restores the formation of fruiting bodies in triple-knockout cells.
  • Fig. S6. Expression of adenylate cyclase and cAMP receptor 1.
  • Fig. S7. PIP3 production and responses in triple-knockout cells.
  • Fig. S8. Myosin IE–GFP localization during phagocytosis.
  • Fig. S9. Myosin IE(A4)–GFP localization during phagocytosis.
  • Table S1. Primers used in this study.
  • Table S2. Plasmids used in this study.
  • Legend for Movies S1 to S11

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Localization of myosin IE–GFP after cAMP stimulation.
  • Movie S2 (.avi format). Localization of myosin IE–GFP after cAMP stimulation in the presence of latrunculin A.
  • Movie S3 (.avi format). Localization of myosin IE–GFP after cAMP stimulation in the presence of LY294002.
  • Movie S4 (.avi format). Localization of myosin IE(E391A)–GFP and myosin IE(E391A)–GFP.
  • Movie S5 (.avi format). Localization of PHcrac-GFP in wild-type and triple-knockout cells after cAMP stimulation.
  • Movie S6 (.avi format). Localization of myosin IE–GFP and PHcrac-RFP during phagocytosis.
  • Movie S7 (.avi format). Localization of myosin IE–GFP and LimEΔcoil-RFP during phagocytosis.
  • Movie S8 (.avi format). Localization of LimEΔcoil-RFP during phagocytosis.
  • Movie S9 (.avi format). Localization of myosin IE–GFP or myosin IE(A4)–GFP during phagocytosis.
  • Movie S10 (.avi format). Localization of YFP–human myosin IF, PHAKT-YFP, and YFP–human myosin IF (K770A, R780A) in COS-7 cells upon EGF treatment.
  • Movie S11 (.avi format). Localization of YFP–human myosin IF and PHAKT-YFP during EGF treatment in the presence of LY294002.

[Download Movies S1 to S11 (Compressed)]

Technical Details

Format: Windows Media Player movies

Size: 4.56 MB (compressed)

Instructions for downloading and decompressing files:

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  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: C.-L. Chen, Y. Wang, H. Sesaki, M. Iijima, Myosin I Links PIP3 Signaling to Remodeling of the Actin Cytoskeleton in Chemotaxis. Sci. Signal. 5, ra10 (2012).

© 2012 American Association for the Advancement of Science

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