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Sci. Signal., 14 February 2012
[DOI: 10.1126/scisignal.2002656]

Measurement of cAMP in an Undergraduate Teaching Laboratory, Using ALPHAscreen Technology

Joseph D. Bartho, Kien Ly, Debbie L. Hay*

School of Biological Sciences, University of Auckland, Auckland, New Zealand.
*Corresponding author: E-mail, dl.hay{at}auckland.ac.nz

Presentation. Signal Transduction in Drug Discovery.

This presentation was used in conjunction with the laboratory exercise for a class of >300 second-year biochemistry students. In addition to describing the lab and providing tips for the students for execution of the laboratory procedures, the presentation includes an overview of the drug discovery process, the use of technologies such as ALPHAscreen in drug discovery, and their advantages over conventional techniques. It also briefly highlights G protein–coupled receptors as therapeutic targets and discusses methods for detecting the intracellular signaling messenger cyclic adenosine monophosphate (cAMP).

References

J. M. Berg, J. L. Tymoczko, L. Stryer, Biochemistry (Freeman, New York, ed. 6, 2007).
J. G. Lombardino, J. A. Lowe III, The role of the medicinal chemist in drug discovery—then and now. Nat. Rev. Drug Discov. 3, 853–862 (2004).
J. P. Overington, B. Al-Lazikani, A. L. Hopkins, How many drug targets are there? Nat. Rev. Drug Discov. 5, 993–996 (2006).
C. Williams, cAMP detection methods in HTS: Selecting the best from the rest. Nat. Rev. Drug Discov. 3, 125–135 (2004).

Technical Details

Format: PDF

Size: 1.27 MB

Requirements: Adobe Acrobat Reader

[Download PDF]

Educational Details

Learning Resource Type: Digital Presentation

Context:
Undergraduate lower division, undergraduate upper division

Intended Users:
Teacher

Intended Educational Use:
Teach, plan, assess

Discipline:
Pharmacology, cell biology, biochemistry

Keywords:
Signal transduction, cell culture, cAMP, G protein–coupled receptor

Citation: J. D. Bartho, K. Ly, D. L. Hay, Measurement of cAMP in an undergraduate teaching laboratory, using ALPHAscreen technology. Sci. Signal. 5, tr1 (2012).

© 2012 American Association for the Advancement of Science


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