Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 6 March 2012
[DOI: 10.1126/scisignal.2001986]

Supplementary Materials for:

Akt and ERK Control the Proliferative Response of Mammary Epithelial Cells to the Growth Factors IGF-1 and EGF Through the Cell Cycle Inhibitor p57Kip2

Devin T. Worster, Tobias Schmelzle, Nicole L. Solimini, Eric S. Lightcap, Bjorn Millard, Gordon B. Mills, Joan S. Brugge,* John G. Albeck

*To whom correspondence should be addressed. E-mail: joan_brugge{at}hms.harvard.edu

This PDF file includes:

  • Fig. S1. HCIF images of p21 under various growth factor conditions.
  • Fig. S2. Covariate single-cell analysis of p57/p21 and pRb.
  • Fig. S3. Immunoblot analysis of p57 depletion.
  • Fig. S4. Proliferative and signaling response to Akt induction.
  • Fig. S5. Immunoblot analysis of changes in p57 protein abundance.
  • Fig. S6. HCIF images of p57 in MCF-12A and PWR-1E epithelial cell lines.
  • Fig. S7. HCIF analysis of p57 regulation in 184A1 mammary epithelial cells.
  • Fig. S8. HCIF analysis of p57 in HUVECS.
  • Fig. S9. HCIF analysis of p57 regulation in U2OS osteosarcoma cells.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.03 MB

Citation: D. T. Worster, T. Schmelzle, N. L. Solimini, E. S. Lightcap, B. Millard, G. B. Mills, J. S. Brugge, J. G. Albeck, Akt and ERK Control the Proliferative Response of Mammary Epithelial Cells to the Growth Factors IGF-1 and EGF Through the Cell Cycle Inhibitor p57Kip2. Sci. Signal. 5, ra19 (2012).

© 2012 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882