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Sci. Signal., 6 March 2012
[DOI: 10.1126/scisignal.2001986]

Supplementary Materials for:

Akt and ERK Control the Proliferative Response of Mammary Epithelial Cells to the Growth Factors IGF-1 and EGF Through the Cell Cycle Inhibitor p57Kip2

Devin T. Worster, Tobias Schmelzle, Nicole L. Solimini, Eric S. Lightcap, Bjorn Millard, Gordon B. Mills, Joan S. Brugge,* John G. Albeck

*To whom correspondence should be addressed. E-mail: joan_brugge{at}hms.harvard.edu

This PDF file includes:

  • Fig. S1. HCIF images of p21 under various growth factor conditions.
  • Fig. S2. Covariate single-cell analysis of p57/p21 and pRb.
  • Fig. S3. Immunoblot analysis of p57 depletion.
  • Fig. S4. Proliferative and signaling response to Akt induction.
  • Fig. S5. Immunoblot analysis of changes in p57 protein abundance.
  • Fig. S6. HCIF images of p57 in MCF-12A and PWR-1E epithelial cell lines.
  • Fig. S7. HCIF analysis of p57 regulation in 184A1 mammary epithelial cells.
  • Fig. S8. HCIF analysis of p57 in HUVECS.
  • Fig. S9. HCIF analysis of p57 regulation in U2OS osteosarcoma cells.

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Citation: D. T. Worster, T. Schmelzle, N. L. Solimini, E. S. Lightcap, B. Millard, G. B. Mills, J. S. Brugge, J. G. Albeck, Akt and ERK Control the Proliferative Response of Mammary Epithelial Cells to the Growth Factors IGF-1 and EGF Through the Cell Cycle Inhibitor p57Kip2. Sci. Signal. 5, ra19 (2012).

© 2012 American Association for the Advancement of Science


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