Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 13 March 2012
[DOI: 10.1126/scisignal.2002351]

Supplementary Materials for:

Regulation of the EGF Transcriptional Response by Endocytic Sorting

Ben Brankatschk, Sven P. Wichert, Shem D. Johnson, Olivier Schaad, Moritz J. Rossner, Jean Gruenberg*

*To whom correspondence should be addressed. E-mail: jean.gruenberg{at}unige.ch

This PDF file includes:

  • Text S1. Analysis of the different conditions of stimulation.
  • Text S2. Quality control for the second NanoString analysis.
  • Text S3. Quality control for the third NanoString analysis.
  • Fig. S1. Quantification of EGFR, as well as phosphorylated EGFR and downstream kinases, after depletion of ESCRT proteins.
  • Fig. S2. Quality controls for microarrays and the first NanoString measurement.
  • Fig. S3. EGF-induced feedback regulators identified in the microarray analysis.
  • Fig. S4. Magnitude and comparison of ESCRT knockdown effects in the microarrays, and network analysis of HRS and TSG101 knockdown effects.
  • Fig. S5. Quantification of EGFR, as well as phosphorylated EGFR and downstream kinases, under different conditions of stimulation with EGF.
  • Fig. S6. Quality controls for the second NanoString experiment and comparison to the first data set.
  • Fig. S7. Quantification of abundance and phosphorylation of EGFR and phosphorylation of downstream kinases after various double knockdowns.
  • Fig. S8. Analysis of EGFR distribution.
  • Fig. S9. EGFR localization after 60 min of EGF stimulation.
  • Fig. S10. Hierarchical clustering of the transcriptional response and of effects in the second NanoString experiment.
  • Fig. S11. Hierarchical clustering of effects in the third NanoString experiment and quality controls.
  • Table S1. Two-way ANOVA analysis of quantification of EGFR and phosphorylated EGFR, MEK1/2, or ERK1/2.
  • Table S2. Microarray analysis of EGF response genes.
  • Table S3. Microarray analysis of knockdown effects.
  • Table S4. Target sequences of the NanoString code sets.
  • Table S5. siRNA target sequences.
  • Table S6. Microarray analysis of all EGF response genes.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 4.27 MB


Citation: B. Brankatschk, S. P. Wichert, S. D. Johnson, O. Schaad, M. J. Rossner, J. Gruenberg, Regulation of the EGF Transcriptional Response by Endocytic Sorting. Sci. Signal. 5, ra21 (2012).

© 2012 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882