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Sci. Signal., 27 March 2012
[DOI: 10.1126/scisignal.2002548]

Supplementary Materials for:

Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast

Noah Dephoure and Steven P. Gygi*

*To whom correspondence should be addressed. E-mail: sgygi{at}hms.harvard.edu

This PDF file includes:

  • Fig. S1. Proteomic analysis of rapamycin-stimulated yeast by hyperplexing.
  • Fig. S2. Quantitative reproducibility and accuracy of the hyperplexing method.
  • Fig. S3. Protein quantification by SILAC.
  • Fig. S4. "3 × 6" hyperplexed analysis of rapamycin-stimulated yeast.
  • Fig. S5. Workflow for identification and quantification of a representative protein from the "3 × 6" experiment.
  • Fig. S6. Delayed response of the yeast proteome to 200 nM rapamycin.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.55 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Log2 ratios of the proteins from the "2 × 6" experiment.
  • Table S2 (Microsoft Excel format). Gene ontology analysis of the regulated proteins from the "2 × 6" and "3 × 6" experiments.
  • Table S3 (Microsoft Excel format). Log2 ratios of the proteins from the "3 × 6" experiment of rapamycin-treated yeast.

[Download Tables S1 to S3 (Compressed)]

Technical Details

Format: Microsoft Excel. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 1.99 MB (compressed); 2.03 MB (decompressed)


Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: N. Dephoure, S. P. Gygi, Hyperplexing: A Method for Higher-Order Multiplexed Quantitative Proteomics Provides a Map of the Dynamic Response to Rapamycin in Yeast. Sci. Signal. 5, rs2 (2012).

© 2012 American Association for the Advancement of Science


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