Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation

Misa Arizono, Hiroko Bannai,* Kyoko Nakamura, Fumihiro Niwa, Masahiro Enomoto, Toru Matsu-ura, Akitoshi Miyamoto, Mark W. Sherwood, Takeshi Nakamura, Katsuhiko Mikoshiba*

*To whom correspondence should be addressed. E-mail: hbannai{at}brain.riken.jp (H.B.); mikosiba{at}brain.riken.jp (K.M.)

This PDF file includes:

• Fig. S1. Spontaneous Ca²⁺ transients in astrocytic processes.
• Fig. S2. GFAP expression in a GCaMP3-transfected cell.
• Fig. S3. Quantification of the likelihood of mGluR5-QD soma-process transition.
• Fig. S4. Visualization of lateral diffusion of overexpressed mGluR5 on the cell surface using FRAP.
• Fig. S5. Effect of mGluR5 C-terminal partial fragment expression on the mGluR5-selective diffusion barrier.
• Legend for Movie S1

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). Spontaneous Ca²⁺ transients in astrocytes.

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© 2012 American Association for the Advancement of Science