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Sci. Signal., 3 April 2012
[DOI: 10.1126/scisignal.2002498]

Supplementary Materials for:

Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation

Misa Arizono, Hiroko Bannai,* Kyoko Nakamura, Fumihiro Niwa, Masahiro Enomoto, Toru Matsu-ura, Akitoshi Miyamoto, Mark W. Sherwood, Takeshi Nakamura, Katsuhiko Mikoshiba*

*To whom correspondence should be addressed. E-mail: hbannai{at}brain.riken.jp (H.B.); mikosiba{at}brain.riken.jp (K.M.)

This PDF file includes:

  • Fig. S1. Spontaneous Ca2+ transients in astrocytic processes.
  • Fig. S2. GFAP expression in a GCaMP3-transfected cell.
  • Fig. S3. Quantification of the likelihood of mGluR5-QD soma-process transition.
  • Fig. S4. Visualization of lateral diffusion of overexpressed mGluR5 on the cell surface using FRAP.
  • Fig. S5. Effect of mGluR5 C-terminal partial fragment expression on the mGluR5-selective diffusion barrier.
  • Legend for Movie S1

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Technical Details

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Size: 589 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Spontaneous Ca2+ transients in astrocytes.

[Download Movie S1 (Compressed)]

Technical Details

Format: Quicktime Movie. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 1.59 MB (compressed); 2.54 MB (decompressed)


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Citation: M. Arizono, H. Bannai, K. Nakamura, F. Niwa, M. Enomoto, T. Matsu-ura, A. Miyamoto, M. W. Sherwood, T. Nakamura, K. Mikoshiba, Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation. Sci. Signal. 5, ra27 (2012).

© 2012 American Association for the Advancement of Science


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