Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 10 April 2012
[DOI: 10.1126/scisignal.2002299]

Supplementary Materials for:

Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca2+ and cAMP Signaling

Debbie Willoughby, Katy L. Everett, Michelle L. Halls, Jonathan Pacheco, Philipp Skroblin, Luis Vaca, Enno Klussmann, Dermot M. F. Cooper*

*To whom correspondence should be addressed. E-mail: dmfc2{at}cam.ac.uk

This PDF file includes:

  • Fig. S1. Constructs used to study AC8 and Orai1 interaction.
  • Fig. S2. Colocalization between Orai1 and cholera toxin B subunit is enhanced following thapsigargin treatment.
  • Fig. S3. Relative levels of GST-tagged AC8 fragments.
  • Fig. S4. Specificity of Orai1 and STIM1 antibodies and Orai1 knockdown.
  • Fig. S5. Spot sequences for peptide array data presented in Fig. 4.
  • Fig. S6. Isolation of the ionomycin-induced, nonspecific Ca2+ entry signal in GCaMP2-AC8– and global GCaMP2–expressing cells.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 645 KB


Citation: D. Willoughby, K. L. Everett, M. L. Halls, J. Pacheco, P. Skroblin, L. Vaca, E. Klussmann, D. M. F. Cooper, Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca2+ and cAMP Signaling. Sci. Signal. 5, ra29 (2012).

© 2012 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882