Quantifying Crosstalk Among Interferon-γ, Interleukin-12, and Tumor Necrosis Factor Signaling Pathways Within a T_H1 Cell Model

David J. Klinke II,* Ning Cheng, Emily Chambers

*To whom correspondence should be addressed. E-mail: david.klinke{at}mail.wvu.edu

This PDF file includes:

• Section 1. Models and modeling.
• Section 2. Model parameters.
• Fig. S1. Convergence of AMCMC results for the cell fate model.
• Fig. S2. AMCMC summary plots for each of the cell fate model parameters.
• Fig. S3. Pairwise comparison of posterior distribution in cell fate rate parameters.
• Fig. S4. Calibration of the cell fate model.
• Fig. S5. The balance between proliferative and apoptotic cell fates depends on time and culture conditions.
• Fig. S6. IL-12 phosphorylates STAT4 in a graded dose-dependent manner in 2D6 cells.
• Fig. S7. Copy number estimation for IL-12Rβ1, IL-12Rβ2, STAT1, and STAT4 in 2D6 cells.
• Fig. S8. Calibration of the cue-signal-response model.
• Fig. S9. IL-12Rβ1 and IL-12Rβ2 exhibited similar dynamics, whereas MCP-1 and IL-6 were unchanged across experimental conditions.
• Fig. S10. 2D6 cells have IFN-γ and TNF-α receptors.
• Fig. S11. Convergence of AMCMC results for the cue-signal-response model.
• Fig. S12. AMCMC summary plots for each of the cue-signal-response model parameters.
• Fig. S13. Pairwise comparison of posterior distribution in rate parameters associated with the cue-signal-response model.
• Fig. S14. Posterior distributions in the rate constants associated with the decline in the amount of pSTAT4.
• Fig. S15. STAT4 and STAT1 are phosphorylated in response to IL-12.
• Fig. S16. IL-12 and IFN-γ phosphorylate STAT1 in primary cells.
• Fig. S17. IL-12 binding enhanced a reduction in the amount of IL-12R.
• Table S1. Values for the cell fate model parameters and initial conditions.
• Table S2. List of cue-signal-response model variables and initial conditions.
• Table S3. Reaction rate laws for the cue-signal-response model.
• Table S4. Differential equations that define the cue-signal-response model.
• Table S5. List of cue-signal-response model parameters and corresponding values.
• References
  

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© 2012 American Association for the Advancement of Science