Studying the Dynamics of SLP-76, Nck, and Vav1 Multimolecular Complex Formation in Live Human Cells with Triple-Color FRET

Maor H. Pauker, Nirit Hassan, Elad Noy, Barak Reicher, Mira Barda-Saad*

*To whom correspondence should be addressed. E-mail: Mira.Barda-Saad{at}biu.ac.il

This PDF file includes:

• Fig. S1. Western blotting analysis of single-labeled cell lines and of positive and negative control cells for 3FRET.
• Fig. S2. Flow cytometric analysis of single-labeled cell lines and of positive and negative control cells for 3FRET.
• Fig. S3. FRET analysis of the interactions between Vav1, Nck, and SLP-76.
• Legend for Movies S1 and S2
• Table S1. Channels used for FRET analysis.
• Table S2. Cell lines used as FRET controls.
• Table S3. 2FRET analysis of the interaction between Nck and Vav1 in the presence or absence of wild-type SLP-76.

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). Dynamics of Nck, Vav1, and wild-type SLP-76 in live T cells.
• Movie S2 (.mov format). Dynamics of Nck, Vav1, and the SLP-76 Y3F mutant in live T cells.

[Download Movies S1 and S2 (Compressed)]

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© 2012 American Association for the Advancement of Science