Genomic Survey of Premetazoans Shows Deep Conservation of Cytoplasmic Tyrosine Kinases and Multiple Radiations of Receptor Tyrosine Kinases

Hiroshi Suga,* Michael Dacre, Alex de Mendoza, Kamran Shalchian-Tabrizi, Gerard Manning,* Iñaki Ruiz-Trillo*

*To whom correspondence should be addressed. E-mail: hiroshi.suga{at}ibe.upf-csic.es (H.S.); manning{at}manninglab.org (G.M.); inaki.ruiz{at}icrea.es (I.R.-T.)

This PDF file includes:

• Fig. S1. Protein domain organizations of all of the TKs of C. owczarzaki and M. vibrans.
• Fig. S2. Phylogenetic position of TKs in the ePK superfamily.
• Fig. S3. ML analysis of the phylogenetic positions of TKs and a test of the hypothesis of the multiple origins of TKs.
• Fig. S4. Bayesian and ML analyses of ePKs without TKs.
• Fig. S5. Statistical tests of the group A position.
• Fig. S6. Statistical test of the group-level clustering of TKs.
• Fig. S7. Phylogenetic tree and domain architectures of holozoan TKs.
• Fig. S8. ML tree and protein domain organizations of cluster I TKs of C. owczarzaki.
• Fig. S9. Diversification of A. queenslandica RTKs in cluster II.
• Fig. S10. An ML tree excluding biased sequences and rapidly evolving sequences.
• Fig. S11. TK families of six holozoans.
• Fig. S12. Phylogenetic tree of CTKs.
• Fig. S13. Protein domain organizations of TKs from three choanoflagellates and their comparison with the M. brevicollis TK repertoire.
• Fig. S14. Comparison of three C. owczarzaki receptor proteins with very similar extracellular regions.
• Fig. S15. Alignment of ePKs.
• Fig. S16. Alignment of holozoan TKs.
• References

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Other Supplementary Material for this manuscript includes the following:

• Sequence data (PDF format)

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© 2012 American Association for the Advancement of Science