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Sci. Signal., 5 June 2012
[DOI: 10.1126/scisignal.2003002]

Supplementary Materials for:

Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors

Kshitiz, Maimon E. Hubbi, Eun Hyun Ahn, John Downey, Junaid Afzal, Deok-Ho Kim, Sergio Rey, Connie Chang, Arnab Kundu, Gregg L. Semenza, Roselle M. Abraham, Andre Levchenko*

*To whom correspondence should be addressed. E-mail: alev{at}jhu.edu

This PDF file includes:

  • Text S1. ODE-based mathematical model.
  • Fig. S1. Substratum rigidity influences single-cell shape and multicell organization of CDCs.
  • Fig. S2. MRS promotes CDC proliferation and endothelial differentiation.
  • Fig. S3. MRS influences cell-substratum and cell-cell interaction dynamics in CDC cultures.
  • Fig. S4. p190RhoGAP mediates cell-cell interaction in aggregate formation by regulating p120-catenin and YAP abundance and localization.
  • Fig. S5. Characterization of p190RhoGAP-shRNA transduction.
  • Fig. S6. CDCs integrate in blood vessels in rat hearts.
  • Fig. S7. Model simulation of cell proliferation and differentiation.
  • Fig. S8. Increase in p190RhoGAP abundance promotes myocardial differentiation of CDCs.
  • Fig. S9. p190RhoGAP sequesters GATA2 in the cytosol.
  • Fig. S10. RhoA influences cell morphology.
  • Legend for Movies S1 to S4

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.11 MB

Other Supplementary Material for this manuscript includes the following:

  • Movies S1 to S4 (.mp4 format). Dynamics of cell network formation from CDC spheroid aggregates.

[Download Movies S1 to S4 (Compressed)]

Technical Details

Format: .mp4. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 3.01 MB (compressed); 3.01 MB (decompressed)


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  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: Kshitiz, M. E. Hubbi, E. H. Ahn, J. Downey, J. Afzal, D.-H. Kim, S. Rey, C. Chang, A. Kundu, G. L. Semenza, R. M. Abraham, A. Levchenko, Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors. Sci. Signal. 5, ra41 (2012).

© 2012 American Association for the Advancement of Science


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