Histone Deacetylases 6 and 9 and Sirtuin-1 Control Foxp3⁺ Regulatory T Cell Function Through Shared and Isoform-Specific Mechanisms

Ulf H. Beier, Liqing Wang, Rongxiang Han, Tatiana Akimova, Yujie Liu, Wayne W. Hancock*

*To whom correspondence should be addressed. E-mail: whancock{at}mail.med.upenn.edu

This PDF file includes:

• Fig. S1. ACY-738 and Tubastatin are HDAC6 inhibitors.
• Fig. S2. Immunofluorescence analysis of T_regs to assess the cytosolic and nuclear localization of Foxp3.
• Fig. S3. Western blotting analysis of T_reg lysates to compare Foxp3 transcription factors.
• Fig. S4. Loss of Sirt1 does not affect conversion to iT_regs.
• Fig. S5. Loss of HDAC9 does not alter TSDR methylation.
• Fig. S6. Purity control of the effector T cells used for the pyrosequencing methylation assay (fig. S5).
• Fig. S7. Combined deletion of Sirt1 and HDAC9 produces minor improvements in T_reg function.
• Fig. S8. Targeting of three HDACs does not improve T_reg function any more than does use of dual HDACi.
• Fig. S9. Proliferation of effector T cells is not affected by HDAC inhibitors.
• Fig. S10. Examples of image processing.
• Table S1. Statistical analysis for Fig. 1C.
• Table S2. Statistical analysis for Fig. 1G.
  

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© 2012 American Association for the Advancement of Science