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Sci. Signal., 3 July 2012
[DOI: 10.1126/scisignal.2002712]

Supplementary Materials for:

Perinuclear Mitochondrial Clustering Creates an Oxidant-Rich Nuclear Domain Required for Hypoxia-Induced Transcription

Abu-Bakr Al-Mehdi, Viktor M. Pastukh, Brad M. Swiger, Darla J. Reed, Mita R. Patel, Gina C. Bardwell, Viktoriya V. Pastukh, Mikhail F. Alexeyev, Mark N. Gillespie*

*To whom correspondence should be addressed. E-mail: mgillesp{at}southalabama.edu

This PDF file includes:

  • Fig. S1. Effect of hypoxia, microtubule inhibition, and dynein motor suppression on microtubule morphology and mitochondrial distribution.
  • Fig. S2. Involvement of ROS in hypoxia-induced perinuclear mitochondrial clustering.
  • Fig. S3. Distribution and dynamic range of nontargeted roGFP.
  • Fig. S4. Effect of perinuclear mitochondrial clustering on DCF-detectable hypoxia-induced ROS production.
  • Fig. S5. Targeting of roGFP to the nucleus.
  • Fig. S6. HREs of the NOS2 and NFKB2 promoters show hypoxia-inducible oxidative base modifications that are attenuated by preventing perinuclear clustering of mitochondria.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.03 MB


Citation: A.-B. Al-Mehdi, V. M. Pastukh, B. M. Swiger, D. J. Reed, M. R. Patel, G. C. Bardwell, V. V. Pastukh, M. F. Alexeyev, M. N. Gillespie, Perinuclear Mitochondrial Clustering Creates an Oxidant-Rich Nuclear Domain Required for Hypoxia-Induced Transcription. Sci. Signal. 5, ra47 (2012).

© 2012 American Association for the Advancement of Science


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