Interference with the PTEN-MAST2 Interaction by a Viral Protein Leads to Cellular Relocalization of PTEN

Elouan Terrien, Alain Chaffotte, Mireille Lafage, Zakir Khan, Christophe Préhaud, Florence Cordier, Catherine Simenel, Muriel Delepierre, Henri Buc, Monique Lafon,* Nicolas Wolff*

*To whom correspondence should be addressed. E-mail: monique.lafon{at}pasteur.fr (M.L.); nicolas.wolff{at}pasteur.fr (N.W.)

This PDF file includes:

• Fig. S1. Intermolecular NOE spectra of MAST2-PDZ in complex with PTEN13-Cter.
• Fig. S2. Cyto13-att disrupts the MAST2-PDZ/PTEN13-Cter complex in vitro.
• Fig. S3. Differences in MAST2-PDZ amide proton chemical shifts between MAST2-PDZ/Cyto13-att and MAST2-PDZ/Cyto13-vir complexes.
• Fig. S4. Intermolecular NOE spectra of MAST2-PDZ in complex with Cyto13-att.
• Fig. S5. Dissociation constant of the MAST2-PDZ dimer measured by analytical ultracentrifugation at various protein concentrations.
• Fig. S6. PTEN localization in human neuronal cells after intracellular delivery of a FITC-conjugated TAT–PTEN13-Cter.
• Table S1. Statistical analysis of MAST2-PDZ/PTEN13-Cter and MAST2-PDZ/Cyto13-att NMR structures.
• Reference
  

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