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Sci. Signal., 14 August 2012
[DOI: 10.1126/scisignal.2002720]

Supplementary Materials for:

Sequential Inter- and Intrasubunit Rearrangements During Activation of Dimeric Metabotropic Glutamate Receptor 1

Veronika Hlavackova, Ulrike Zabel, Daniela Frankova, Julia Bätz, Carsten Hoffmann, Laurent Prezeau, Jean-Philippe Pin,* Jaroslav Blahos,* Martin J. Lohse*

*To whom correspondence should be addressed. E-mail: lohse{at}toxi.uni-wuerzburg.de (M.J.L.); blahos{at}img.cas.cz (J.B.); Jean-Philippe.Pin{at}igf.cnrs.fr (J.-P.P.)

This PDF file includes:

  • Fig. S1. mGluR1 constructs for intersubunit FRET are present on the cell surface.
  • Fig. S2. Uncoupling of the VFT domain of the A-sensor subunit abolishes agonist-induced changes in FRET.
  • Fig. S3. Formation of heterodimers with the A-sensor.
  • Table S1. Positioning of the fluorophores within the mGlu1-based receptor FRET sensors.
  • Table S2. Values for the Hill coefficient (h) and 95% confidence interval for concentration-response curves.

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Technical Details

Format: Adobe Acrobat PDF

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Citation: V. Hlavackova, U. Zabel, D. Frankova, J. Bätz, C. Hoffmann, L. Prezeau, J.-P. Pin, J. Blahos, M. J. Lohse, Sequential Inter- and Intrasubunit Rearrangements During Activation of Dimeric Metabotropic Glutamate Receptor 1. Sci. Signal. 5, ra59 (2012).

© 2012 American Association for the Advancement of Science


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