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Sci. Signal., 2 October 2012
[DOI: 10.1126/scisignal.2002962]

Supplementary Materials for:

The Rho Exchange Factors Vav2 and Vav3 Control a Lung Metastasis–Specific Transcriptional Program in Breast Cancer Cells

Carmen Citterio, Mauricio Menacho-Márquez, Ramón García-Escudero, Romain M. Larive, Olga Barreiro, Francisco Sánchez-Madrid, Jesús M. Paramio, Xosé R. Bustelo*

*To whom correspondence should be addressed. E-mail: xbustelo{at}

This PDF file includes:

  • Fig. S1. Human tumors show differential abundance of Rho GEF transcripts.
  • Fig. S2. Abundance of Vav family mRNAs and proteins in cell lines used in this study.
  • Fig. S3. Vav proteins control a large transcriptomal program in breast cancer cells.
  • Fig. S4. Regulation of the breast cancer cell transcriptome by Vav proteins.
  • Fig. S5. Vav proteins control the breast cancer cell transcriptome through common, synergistic, and Vav family member–specific routes.
  • Fig. S6. Generation of knockdown cell lines for Vav family–dependent targets.
  • Fig. S7. Vav-dependent mRNA signatures have prognostic value in breast cancer.
  • Table S1. Differential abundance of Rho GEFs in human breast cancer subtypes.
  • Table S2. Abundance of Vav2 and Vav3 mRNAs in the breast cancer luminal A and B subtypes.
  • Table S3. Cell lines used in this study.
  • Table S4. Metastasis of 4T1 knockdown cell clones in recipient mice.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.45 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Transcriptomal changes in 4T1 cells lacking Vav2 and Vav3.
  • Table S6 (Microsoft Excel format). The Vav2/Vav3-dependent transcriptome associated to the metastatic potential of the 4T1 cells used in this study.
  • Table S7 (Microsoft Excel format). Overexpressed and repressed genes from table S5 associated with human cancer clinical outcome.
  • Table S8 (Microsoft Excel format). Genes belonging to the Vav2/Vav3 lung metastasis signature.

[Download Tables S5 to S8]

Technical Details

Format: Microsoft Excel

Size: 1.29 MB

Citation: C. Citterio, M. Menacho-Márquez, R. García-Escudero, R. M. Larive, O. Barreiro, F. Sánchez-Madrid, J. M. Paramio, X. R. Bustelo, The Rho Exchange Factors Vav2 and Vav3 Control a Lung Metastasis–Specific Transcriptional Program in Breast Cancer Cells. Sci. Signal. 5, ra71 (2012).

© 2012 American Association for the Advancement of Science

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