TWEAK and cIAP1 Regulate Myoblast Fusion Through the Noncanonical NF-κB Signaling Pathway

Emeka K. Enwere, Janelle Holbrook, Rim Lejmi-Mrad, Jennifer Vineham, Kristen Timusk, Baktharaman Sivaraj, Methvin Isaac, David Uehling, Rima Al-awar, Eric LaCasse, Robert G. Korneluk*

*To whom correspondence should be addressed. E-mail: bob{at}arc.cheo.ca

This PDF file includes:

• Synthesis, structure, and characterization of the SMC OICR720.
• Fig. S1. SMC triggers the loss of cIAP1 from primary myoblasts.
• Fig. S2. The abundances of mitochondrial markers are not affected by loss of cIAP1.
• Fig. S3. Loss of cIAP1 inhibits myoblast fusion in C2C12 cells.
• Fig. S4. Loss of cIAP1 leads to activation of NF-κB pathways.
• Fig. S5. TWEAK activates noncanonical NF-κB signaling and myoblast fusion.
• Fig. S6. TWEAK activates canonical NF-κB signaling only at high concentrations.
• Fig. S7. NEMO is not required for TWEAK-enhanced myoblast fusion.
• Fig. S8. MEK1/2 inhibitors attenuate basal and TWEAK-enhanced myoblast fusion.
• Table S1. Sequences of siRNA duplexes.
• Movie S1 legend
  

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Time-lapse imaging of myoblast fusion.

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© 2012 American Association for the Advancement of Science