Cycles of Ubiquitination and Deubiquitination Critically Regulate Growth Factor–Mediated Activation of Akt Signaling

Wei-Lei Yang, Guoxiang Jin, Chien-Feng Li, Yun Seong Jeong, Asad Moten, Dazhi Xu, Zizhen Feng, Wei Chen, Zhen Cai, Bryant Darnay, Wei Gu, Hui-Kuan Lin*

*To whom correspondence should be addressed. E-mail: hklin{at}mdanderson.org

This PDF file includes:

• Fig. S1. CYLD, but not USP7 and USP22, promotes deubiquitination of Akt.
• Fig. S2. CYLD deubiquitinates and interacts with Akt isoforms.
• Fig. S3. Phosphorylation of Akt does not affect its interaction with CYLD.
• Fig. S4. Cyld^–/– MEFs show enhanced basal membrane localization of Akt.
• Fig. S5. The role of PI3K activity in the membrane recruitment of Akt in wild-type and Cyld^–/– MEFs.
• Fig. S6. CYLD deficiency enhances cell proliferation in primary MEFs and breast cancer cells.
• Table S1. Quantification of the indicated immunoblotting signals.

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© 2013 American Association for the Advancement of Science