Systems-Level Analysis of Proteolytic Events in Increased Vascular Permeability and Complement Activation in Skin Inflammation

Ulrich auf dem Keller, Anna Prudova, Ulrich Eckhard, Barbara Fingleton, Christopher M. Overall*

*To whom correspondence should be addressed. E-mail: chris.overall{at}ubc.ca

This PDF file includes:

• Fig. S1. iTRAQ labeling scheme for TAILS experiments.
• Fig. S2. Skin inflammatory proteome (iTRAQ-preTAILS).
• Fig. S3. Distribution of acetylated and non-acetylated N termini with preferred residues.
• Fig. S4. Dynamics of actin acetylation revealed by iTRAQ-TAILS in vivo.
• Fig. S5. Consensus sequence for cyclized N termini after pyroglutamate formation.
• Fig. S6. Original mature N terminus of stromelysin-1 (MMP3) identified by iTRAQTAILS.
• Fig. S7. Original mature N terminus of S100A9 identified by iTRAQ-TAILS.
• Fig. S8. Densitometric analysis of Western blotting analyses shown in Fig. 5, B and C.
• Fig. S9. Western blotting analysis of haptoglobin abundance in livers of Mmp2^–/– mice and wild-type controls upon mustard oil–induced skin inflammation.
• Fig. S10. Prediction of signal peptide removal for mannose-binding protein A (MBL1).

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Other Supplementary Material for this manuscript includes the following:

• Tables S1 to S19 (Microsoft Excel format).
• Supplementary files 1 to 8 showing spectra (.pdf format).

© 2013 American Association for the Advancement of Science