Ca²⁺-Dependent Phosphorylation of Ca²⁺ Cycling Proteins Generates Robust Rhythmic Local Ca²⁺ Releases in Cardiac Pacemaker Cells

Syevda Sirenko, Dongmei Yang, Yue Li, Alexey E. Lyashkov, Yevgeniya O. Lukyanenko, Edward G. Lakatta, Tatiana M. Vinogradova*

*To whom correspondence should be addressed. E-mail: vinogradovat{at}grc.nia.nih.gov

This PDF file includes:

• Fig. S1. Local Ca²⁺ release characteristics are Ca²⁺-dependent and differ in sinoatrial node cells and ventricular myocytes.
• Fig. S2. The size of spontaneous local Ca²⁺releases delineates their periodicity.
• Fig. S3. High cytosolic Ca²⁺ concentrations eliminate local Ca²⁺ releases in sinoatrial node cells.
• Fig. S4. Increased cytosolic Ca²⁺ concentrations increase phosphorylation of PLB at Thr¹⁷ in sinoatrial node cells, but not in ventricular myocytes.
• Fig. S5. Increased cytosolic Ca²⁺ concentrations do not change the phosphorylation of PLB at Thr¹⁷ in ventricular myocytes.
• Fig. S6. Phosphorylated RyR immunolabeling in ventricular myocytes is not Ca²⁺- dependent.
• Fig. S7. Effects of PKI and AIP on the characteristics of local Ca²⁺ releases in permeabilized sinoatrial node cells.

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