Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 12 February 2013
[DOI: 10.1126/scisignal.2003087]

Supplementary Materials for:

ERK-Mediated Phosphorylation of Fibroblast Growth Factor Receptor 1 on Ser777 Inhibits Signaling

Malgorzata Zakrzewska, Ellen Margrethe Haugsten, Beata Nadratowska-Wesolowska, Angela Oppelt, Barbara Hausott, Yixin Jin, Jacek Otlewski, Jørgen Wesche, Antoni Wiedlocha*

*To whom correspondence should be addressed. E-mail: Antoni.Wiedlocha{at}

This PDF file includes:

  • Fig. S1. In vitro phosphorylation assay with the recombinant C-terminal tail of FGFR1 and Akt or MEK1.
  • Fig. S2. Effect of U0126 on ERK1/2 activity.
  • Fig. S3. Effect of MEK inhibitors on FGFR1 activity in the presence or absence of brefeldin A or cycloheximide.
  • Fig. S4. The effect of siRNA-mediated knockdown of ERK1/2 and GRB2 on FGFR1 activity.
  • Fig. S5. Effect of the phosphorylation status of FGFR1 Ser777 on cell proliferation and migration in additional clones of stably transfected U2OS cells.
  • Fig. S6. Correlation between organism complexity and tyrosine, serine, and threonine contents in the cytoplasmic region of FGFR1 proteins.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 3.20 MB

Citation: M. Zakrzewska, E. M. Haugsten, B. Nadratowska-Wesolowska, A. Oppelt, B. Hausott, Y. Jin, J. Otlewski, J. Wesche, A. Wiedlocha, ERK-Mediated Phosphorylation of Fibroblast Growth Factor Receptor 1 on Ser777 Inhibits Signaling. Sci. Signal. 6, ra11 (2013).

© 2013 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882