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Sci. Signal., 5 March 2013
[DOI: 10.1126/scisignal.2003398]

Supplementary Materials for:

The Small GTPase ARF6 Stimulates β-Catenin Transcriptional Activity During WNT5A-Mediated Melanoma Invasion and Metastasis

Allie H. Grossmann, Jae Hyuk Yoo, James Clancy, Lise K. Sorensen, Alanna Sedgwick, Zongzhong Tong, Kirill Ostanin, Aaron Rogers, Kenneth F. Grossmann, Sheryl R. Tripp, Kirk R. Thomas, Crislyn D'Souza-Schorey, Shannon J. Odelberg,* Dean Y. Li*

*To whom correspondence should be addressed. E-mail:{at} (D.Y.L.); sodelber{at} (S.J.O.)

This PDF file includes:

  • Fig. S1. ARF6 knockdown does not alter total N-cadherin protein at the plasma membrane.
  • Fig. S2. Quantification of immunoblots.
  • Fig. S3. ARF6 knockdown drives β-catenin from the nucleus to N-cadherin.
  • Fig. S4. ARF6, junctional β-catenin, and the canonical destruction complex.
  • Fig. S5. SLIT2-ROBO1 and SecinH3 inhibit ARF6 activation.
  • Fig. S6. Time course of β-catenin relocalization after ARF6 inhibition.
  • Fig. S7. ARF6-dependent β-catenin transactivation and relative WNT production in melanoma cell lines.
  • Fig. S8. WNT2 knockdown reduces ARF6 activation.
  • Fig. S9. FZD4 knockdown reduces ARF6 activation.
  • Fig. S10. WNT5A, ARF6, and β-catenin signaling in multiple human melanoma cell lines.
  • Fig. S11. Cytohesin knockdown does not reduce ARF6 activation.
  • Fig. S12. β-Catenin immunostaining of LOX melanoma xenograft tumors.
  • Table S1. siRNA and shRNA sequences.
  • Table S2. Primer sequences for RT-PCR.

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Citation: A. H. Grossmann, J. H. Yoo, J. Clancy, L. K. Sorensen, A. Sedgwick, Z. Tong, K. Ostanin, A. Rogers, K. F. Grossmann, S. R. Tripp, K. R. Thomas, C. D'Souza-Schorey, S. J. Odelberg, D. Y. Li, The Small GTPase ARF6 Stimulates β-Catenin Transcriptional Activity During WNT5A-Mediated Melanoma Invasion and Metastasis. Sci. Signal. 6, ra14 (2013).

© 2013 American Association for the Advancement of Science

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