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Sci. Signal., 16 April 2013
[DOI: 10.1126/scisignal.2003572]

Supplementary Materials for:

Phosphoproteomic Analysis Implicates the mTORC2-FoxO1 Axis in VEGF Signaling and Feedback Activation of Receptor Tyrosine Kinases

Guanglei Zhuang, Kebing Yu, Zhaoshi Jiang, Alicia Chung, Jenny Yao, Connie Ha, Karen Toy, Robert Soriano, Benjamin Haley, Elizabeth Blackwood, Deepak Sampath, Carlos Bais, Jennie R. Lill, Napoleone Ferrara*

*Corresponding author. E-mail: nferrara{at}ucsd.edu

This PDF file includes:

  • Fig. S1. KinomeView profiling of endothelial cells.
  • Fig. S2. Quality control of phosphoproteomic experiments.
  • Fig. S3. Regulation of PI3K-mTORC2-Akt-FoxO1 axis by VEGF.
  • Fig. S4. Different classes of small-molecule inhibitors used in the study.
  • Fig. S5. VEGF signaling mechanisms to regulate phosphorylation.
  • Fig. S6. mTOR kinase inhibitors in endothelial RTK reprogramming.
  • Fig. S7. Feedback activation of endothelial RTKs.
  • Fig. S8. PI3K inhibitors in endothelial RTK reprogramming.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.34 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). The complete data set for identified phosphopeptides.
  • Table S2 (Microsoft Excel format). VEGF-regulated phosphopeptides and membership values in fuzzy c-means clustering.
  • Table S3 (Microsoft Excel format). Gene ontology and pathway analysis of the VEGF-regulated proteins.
  • Table S4 (Microsoft Excel format). Gene list and siRNA sequences for RNAi screen.
  • Table S5 (Microsoft Excel format). Kinase enrichment analysis of the VEGF-regulated phosphoproteins.
  • Table S6 (Microsoft Excel format). Microarray analysis of HUVECs stimulated with VEGF.
  • Table S7 (Microsoft Excel format). NanoString nCounter analysis of HUVECs stimulated with VEGF.
  • Table S8 (Microsoft Excel format). Microarray analysis of HUVECs treated with mTOR kinase inhibitors.

Citation: G. Zhuang, K. Yu, Z. Jiang, A. Chung, J. Yao, C. Ha, K. Toy, R. Soriano, B. Haley, E. Blackwood, D. Sampath, C. Bais, J. R. Lill, N. Ferrara, Phosphoproteomic Analysis Implicates the mTORC2-FoxO1 Axis in VEGF Signaling and Feedback Activation of Receptor Tyrosine Kinases. Sci. Signal. 6, ra25 (2013).

© 2013 American Association for the Advancement of Science


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