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Sci. Signal., 23 April 2013
[DOI: 10.1126/scisignal.2003664]

Supplementary Materials for:

Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response

Vincentius A. Halim, Mónica Alvarez-Fernández, Yan Juan Xu, Melinda Aprelia, Henk W. P. van den Toorn, Albert J. R. Heck, Shabaz Mohammed, René H. Medema*

*Corresponding author. E-mail: r.medema{at}nki.nl

This PDF file includes:

  • Fig. S1. Schematic of the checkpoint recovery assay.
  • Fig. S2. In-depth proteomics data analysis.
  • Fig. S3. Enrichment of candidates with particular functions.
  • Fig. S4. Astrin affects the p53/MDM2 feedback loop.
  • Legends for tables S1 to S8

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 345 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (PDF and Excel format). All quantified phosphopeptides.
  • Table S2 (PDF and Excel format). All identified peptides (unphosphorylated).
  • Table S3 (PDF and Excel format). All identified proteins.
  • Table S4 (PDF and Excel format). Comparing phosphosites that are phosphorylated by PLK1 and phosphosites of Polo box interactors.
  • Table S5 (PDF and Excel format). Protein with changes on phosphopeptide abundance (at least in two experiments).
  • Table S6 (PDF and Excel format). Comparing phosphosites that are phosphorylated by PLK1 during recovery and also phosphorylated with pSQ/TQ motif upon DNA damage.
  • Table S7 (PDF and Excel format). Primary siRNA screen to identify proteins involved in recovery and unperturbed mitotic entry control.
  • Table S8 (PDF and Excel format). Secondary screen for siRNA pool deconvolution.

[Download Tables S1 to S8 in PDF format]

[Download Tables S1 to S8 in Excel format]

Technical Details

Format: Adobe Acrobat PDF; Microsoft Excel

Size: 5.28 MB (PDF); 11.4 MB Excel unzipped

Update 26 April 2013

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) through the PRIDE partner repository (1) with the dataset identifier PXD000222 and DOI 10.6019/PXD000222.

1. J. A. Vizcaíno, R. G. Côté, A. Csordas, J. A. Dianes, A. Fabregat, J. M. Foster, J. Griss, E. Alpi, M. Birim, J. Contell, G. O'Kelly, A. Schoenegger, D. Ovelleiro, Y. Pérez-Riverol, F. Reisinger, D. Ríos. R. Wang, H. Hermjakob. The Proteomics Identifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 41,D1063-9 (2013).


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Citation: V. A. Halim, M. Alvarez-Fernández, Y. J. Xu, M. Aprelia, H. W. P. van den Toorn, A. J. R. Heck, S. Mohammed, R. H. Medema, Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response. Sci. Signal. 6, rs9 (2013).

© 2013 American Association for the Advancement of Science


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