Sci. STKE, 28 March 2000
Calcium Coordinating Calcium Release
Several mechanisms exist for releasing intracellular stores of calcium: activation of inositol trisphosphate (IP3) receptors, activation of ryanodine receptors, and stimulation of Ca2+ release by nicotinic acid adenine dinucleotide phosphate (NAADP+). Santella et al. studied calcium fluxes in starfish oocytes in response to injected caged NAADP+. Photolysis of the caged NAADP+ led to an increase in intracellular calcium [Ca2+]i that was partially blocked by the addition of both heparin (to block Ca2+ release from IP3 receptors) and 8-NH2-cyclic ADPribose (to block Ca2+ release from ryanodine receptors). The authors suggest that NAADP+ initiates an increase in [Ca2+]i that triggers further release from IP3 and ryanodine receptor-operated stores. Additionally, NAADP+ stimulated an increase in [Ca2+]i from intracellular stores in immature oocytes, but the NAADP+-stimulated increase in [Ca2+]i in mature oocytes was derived from extracellular sources of calcium, suggesting that the NAADP+-sensitive compartment fused with the plasma membrane as a consequence of oocyte maturation.
Santella, L., Kyozuka, K., Genazzani, A.A., De Riso, L., and Carafoli, E. (2000) Nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release. J. Biol. Chem. 275: 8301-8306. [Abstract] [Full Text]
Citation: Coordinating Calcium Release. Sci. STKE 2000, tw3 (2000).
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