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Sci. Signal., 15 January 2013
Vol. 6, Issue 258, p. rs2
Taking a Snapshot of Inflammation
Studies of inflammatory mediators, such as proteases and their inhibitors, often involve in vitro analyses or focus on a single target in vivo. However, given the complex interplay among all of the players in the inflammatory response, a system-wide analysis would provide a better understanding of how each factor works in its natural environment. With their approach of quantitatively labeling the N termini of proteins (both uncleaved and after protease-mediated cleavage) from inflamed mouse skin tissue, auf dem Keller et al. provide a snapshot of the inflammatory response in vivo. Their analysis identified previously uncharacterized cleavage events within the complement cascade and highlighted a role for matrix metalloproteinase 2, which contributed to increased blood vessel permeability during inflammation. Together, these data provide a system-wide proteolytic fingerprint of skin inflammation and generate functional hypotheses that warrant further investigation.
Citation: U. auf dem Keller, A. Prudova, U. Eckhard, B. Fingleton, C. M. Overall, Systems-Level Analysis of Proteolytic Events in Increased Vascular Permeability and Complement Activation in Skin Inflammation. Sci. Signal.6, rs2 (2013).