Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Sci. Signal., 23 April 2013
Vol. 6, Issue 272, p. rs9
Uncovering the Damage Recovery Phosphoproteome
DNA damage triggers a signaling cascade that leads to cell cycle arrest and DNA repair or cell death. Halim et al. combined phosphoproteomics and RNA interference to elucidate the molecular events associated with reentry into the cell cycle after DNA damage. They found 10 differentially phosphorylated proteins that were necessary for reentry into mitosis of cells recovering from -irradiation or doxorubicin treatment but not for cell cycle progression of unperturbed cells. Astrin, a protein associated with the mitotic spindle, promoted the expression of critical cell cycle genes by reducing the abundance of p53, a transcription factor that contributes to cell cycle arrest and apoptosis. Thus, this study revealed avenues to explore how cells maintain cell cycle competence during cell cycle arrest.
Citation: V. A. Halim, M. Alvarez-Fernández, Y. J. Xu, M. Aprelia, H. W. P. van den Toorn, A. J. R. Heck, S. Mohammed, R. H. Medema, Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response. Sci. Signal.6, rs9 (2013).
The editors suggest the following Related Resources on Science sites:
In Science Signaling
Guy Riddihough (28 May 2013) Sci. Signal.6 (277), ec126.
[DOI: 10.1126/scisignal.2004356] |Abstract »
Jordi Carreras Puigvert, Louise von Stechow, Ramakrishnaiah Siddappa, Alex Pines, Mahnoush Bahjat, Lizette C. J. M. Haazen, Jesper V. Olsen, Harry Vrieling, John H. N. Meerman, Leon H. F. Mullenders, Bob van de Water, and Erik H. J. Danen (22 January 2013) Sci. Signal.6 (259), ra5.
[DOI: 10.1126/scisignal.2003208] |Editor's Summary »|Abstract »|Full Text »|PDF »|Supplementary Materials »