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Sci. Signal., 23 April 2013
Vol. 6, Issue 272, p. rs9
Uncovering the Damage Recovery Phosphoproteome
DNA damage triggers a signaling cascade that leads to cell cycle arrest and DNA repair or cell death. Halim et al. combined phosphoproteomics and RNA interference to elucidate the molecular events associated with reentry into the cell cycle after DNA damage. They found 10 differentially phosphorylated proteins that were necessary for reentry into mitosis of cells recovering from -irradiation or doxorubicin treatment but not for cell cycle progression of unperturbed cells. Astrin, a protein associated with the mitotic spindle, promoted the expression of critical cell cycle genes by reducing the abundance of p53, a transcription factor that contributes to cell cycle arrest and apoptosis. Thus, this study revealed avenues to explore how cells maintain cell cycle competence during cell cycle arrest.
Citation: V. A. Halim, M. Alvarez-Fernández, Y. J. Xu, M. Aprelia, H. W. P. van den Toorn, A. J. R. Heck, S. Mohammed, R. H. Medema, Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response. Sci. Signal.6, rs9 (2013).
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