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Sci. Signal., 11 November 2008
Vol. 1, Issue 45, p. ra12
[DOI: 10.1126/scisignal.2000037]


Editor's Summary

Finding the Right Candidate
A genome-wide RNAi screen in human colon cancer cells, followed by two additional validation steps, reveals new components of the Wnt pathway. Combining RNAi analysis with protein-protein interaction data provides a powerful approach that not only identifies new players in a signaling pathway, but also provides functional insight about the modulators, leading to the generation of testable hypotheses.

Citation: M. B. Major, B. S. Roberts, J. D. Berndt, S. Marine, J. Anastas, N. Chung, M. Ferrer, X. Yi, C. L. Stoick-Cooper, P. D. von Haller, L. Kategaya, A. Chien, S. Angers, M. MacCoss, M. A. Cleary, W. T. Arthur, R. T. Moon, New Regulators of Wnt/β-Catenin Signaling Revealed by Integrative Molecular Screening. Sci. Signal. 1, ra12 (2008).

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Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway.
B. W. Miller, G. Lau, C. Grouios, E. Mollica, M. Barrios-Rodiles, Y. Liu, A. Datti, Q. Morris, J. L. Wrana, and L. Attisano (2014)
Mol Syst Biol 5, 315
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Sall1 balances self-renewal and differentiation of renal progenitor cells.
J. M. Basta, L. Robbins, S. M. Kiefer, D. Dorsett, and M. Rauchman (2014)
Development 141, 1047-1058
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{beta}-Arrestin Promotes Wnt-induced Low Density Lipoprotein Receptor-related Protein 6 (Lrp6) Phosphorylation via Increased Membrane Recruitment of Amer1 Protein.
V. Kriz, V. Pospichalova, J. Masek, M. B. C. Kilander, J. Slavik, K. Tanneberger, G. Schulte, M. Machala, A. Kozubik, J. Behrens, et al. (2014)
J. Biol. Chem. 289, 1128-1141
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Protein Kinase PKN1 Represses Wnt/{beta}-Catenin Signaling in Human Melanoma Cells.
R. G. James, K. A. Bosch, R. M. Kulikauskas, P. T. Yang, N. C. Robin, R. A. Toroni, T. L. Biechele, J. D. Berndt, P. D. von Haller, J. K. Eng, et al. (2013)
J. Biol. Chem. 288, 34658-34670
   Abstract »    Full Text »    PDF »
Targeting Wnt Pathways in Disease.
Z. F. Zimmerman, R. T. Moon, and A. J. Chien (2012)
Cold Spring Harb Perspect Biol 4, a008086
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Functional genomics identifies therapeutic targets for MYC-driven cancer.
M. Toyoshima, H. L. Howie, M. Imakura, R. M. Walsh, J. E. Annis, A. N. Chang, J. Frazier, B. N. Chau, A. Loboda, P. S. Linsley, et al. (2012)
PNAS 109, 9545-9550
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Wnt/{beta}-catenin signaling is differentially regulated by G{alpha} proteins and contributes to fibrous dysplasia.
J. B. Regard, N. Cherman, D. Palmer, S. A. Kuznetsov, F. S. Celi, J.-M. Guettier, M. Chen, N. Bhattacharyya, J. Wess, S. R. Coughlin, et al. (2011)
PNAS 108, 20101-20106
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Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal-Regulated Kinase Signaling.
A. A. Friedman, G. Tucker, R. Singh, D. Yan, A. Vinayagam, Y. Hu, R. Binari, P. Hong, X. Sun, M. Porto, et al. (2011)
Science Signaling 4, rs10
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ASPM regulates Wnt signaling pathway activity in the developing brain.
J. J. Buchman, O. Durak, and L.-H. Tsai (2011)
Genes & Dev. 25, 1909-1914
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Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/{beta}-catenin signaling.
J. D. Berndt, A. Aoyagi, P. Yang, J. N. Anastas, L. Tang, and R. T. Moon (2011)
J. Cell Biol. 194, 737-750
   Abstract »    Full Text »    PDF »
The Ubiquitin-Specific Protease USP34 Regulates Axin Stability and Wnt/{beta}-Catenin Signaling.
T. T. H. Lui, C. Lacroix, S. M. Ahmed, S. J. Goldenberg, C. A. Leach, A. M. Daulat, and S. Angers (2011)
Mol. Cell. Biol. 31, 2053-2065
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Jerky/Earthbound facilitates cell-specific Wnt/Wingless signalling by modulating {beta}-catenin-TCF activity.
H. Benchabane, N. Xin, A. Tian, B. P. Hafler, K. Nguyen, A. Ahmed, and Y. Ahmed (2011)
EMBO J. 30, 1444-1458
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The GDNF Target Vsnl1 Marks the Ureteric Tip.
R. Ola, M. Jakobson, J. Kvist, N. Perala, S. Kuure, K.-H. Braunewell, D. Bridgewater, N. D. Rosenblum, D. Chilov, T. Immonen, et al. (2011)
J. Am. Soc. Nephrol. 22, 274-284
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Exosome release of {beta}-catenin: a novel mechanism that antagonizes Wnt signaling.
A. Chairoungdua, D. L. Smith, P. Pochard, M. Hull, and M. J. Caplan (2010)
J. Cell Biol. 190, 1079-1091
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Inactivation of LEF1 in T-cell acute lymphoblastic leukemia.
A. Gutierrez, T. Sanda, W. Ma, J. Zhang, R. Grebliunaite, S. Dahlberg, D. Neuberg, A. Protopopov, S. S. Winter, R. S. Larson, et al. (2010)
Blood 115, 2845-2851
   Abstract »    Full Text »    PDF »
Transcription-Based Reporters of Wnt/{beta}-Catenin Signaling.
T. L. Biechele, A. M. Adams, and R. T. Moon (2009)
Cold Spring Harb Protoc 2009, pdb.prot5223
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Bruton's Tyrosine Kinase Revealed as a Negative Regulator of Wnt-{beta}-Catenin Signaling.
R. G. James, T. L. Biechele, W. H. Conrad, N. D. Camp, D. M. Fass, M. B. Major, K. Sommer, X. Yi, B. S. Roberts, M. A. Cleary, et al. (2009)
Science Signaling 2, ra25
   Abstract »    Full Text »    PDF »
Integrative Analysis of Genome-Wide RNA Interference Screens.
J. D. Berndt, T. L. Biechele, R. T. Moon, and M. B. Major (2009)
Science Signaling 2, pt4
   Abstract »    Full Text »    PDF »
2008: Signaling Breakthroughs of the Year.
E. M. Adler (2009)
Science Signaling 2, eg1
   Abstract »    Full Text »    PDF »
Science Signaling Podcast: 11 November 2008.
M. B. Major, R. T. Moon, and A. M. VanHook (2008)
Science Signaling 1, pc11
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