Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 24 November 2009
Vol. 2, Issue 98, p. ra77
[DOI: 10.1126/scisignal.2000466]

RESEARCH ARTICLES

Editor's Summary

No Assembly Required
The second messenger inositol trisphosphate (IP3) acts on IP3 receptors (IP3Rs) in the endoplasmic reticulum (ER) membrane to elicit Ca2+ release from intracellular stores, enabling the generation of intracellular Ca2+ signals from extracellular messengers. The regulation of these Ca2+ signals depends on the clustered organization of IP3R. Using optical techniques capable of recording single-channel IP3R activity in intact mammalian cells, Smith et al. explored the dynamics of IP3-dependent Ca2+ signals and concluded that local Ca2+ signals (known as puffs) arise from preassembled clusters of IP3R rather than depending on rapid and dynamic clustering in response to the IP3 signal itself.

Citation: I. F. Smith, S. M. Wiltgen, J. Shuai, I. Parker, Ca2+ Puffs Originate from Preestablished Stable Clusters of Inositol Trisphosphate Receptors. Sci. Signal. 2, ra77 (2009).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cooperative and Stochastic Calcium Releases from Multiple Calcium Puff Sites Generate Calcium Microdomains in Intact HeLa Cells.
H. Nakamura, H. Bannai, T. Inoue, T. Michikawa, M. Sano, and K. Mikoshiba (2012)
J. Biol. Chem. 287, 24563-24572
   Abstract »    Full Text »    PDF »
Differential Distribution, Clustering, and Lateral Diffusion of Subtypes of the Inositol 1,4,5-Trisphosphate Receptor.
E. Pantazaka and C. W. Taylor (2011)
J. Biol. Chem. 286, 23378-23387
   Abstract »    Full Text »    PDF »
Calcium Oscillations.
G. Dupont, L. Combettes, G. S. Bird, and J. W. Putney (2011)
Cold Spring Harb Perspect Biol 3, a004226
   Abstract »    Full Text »    PDF »
Seeing is believing! Imaging Ca2+-signalling events in living cells.
J. G. McGeown (2010)
Exp Physiol 95, 1049-1060
   Abstract »    Full Text »    PDF »
Perspectives on: Local calcium signaling: Recording single-channel activity of inositol trisphosphate receptors in intact cells with a microscope, not a patch clamp.
I. Parker and I. F. Smith (2010)
J. Gen. Physiol. 136, 119-127
   Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882