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Sci. Signal., 24 November 2009
Vol. 2, Issue 98, p. ra77
[DOI: 10.1126/scisignal.2000466]


Editor's Summary

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The second messenger inositol trisphosphate (IP3) acts on IP3 receptors (IP3Rs) in the endoplasmic reticulum (ER) membrane to elicit Ca2+ release from intracellular stores, enabling the generation of intracellular Ca2+ signals from extracellular messengers. The regulation of these Ca2+ signals depends on the clustered organization of IP3R. Using optical techniques capable of recording single-channel IP3R activity in intact mammalian cells, Smith et al. explored the dynamics of IP3-dependent Ca2+ signals and concluded that local Ca2+ signals (known as puffs) arise from preassembled clusters of IP3R rather than depending on rapid and dynamic clustering in response to the IP3 signal itself.

Citation: I. F. Smith, S. M. Wiltgen, J. Shuai, I. Parker, Ca2+ Puffs Originate from Preestablished Stable Clusters of Inositol Trisphosphate Receptors. Sci. Signal. 2, ra77 (2009).

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Cooperative and Stochastic Calcium Releases from Multiple Calcium Puff Sites Generate Calcium Microdomains in Intact HeLa Cells.
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J. Biol. Chem. 287, 24563-24572
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Differential Distribution, Clustering, and Lateral Diffusion of Subtypes of the Inositol 1,4,5-Trisphosphate Receptor.
E. Pantazaka and C. W. Taylor (2011)
J. Biol. Chem. 286, 23378-23387
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Calcium Oscillations.
G. Dupont, L. Combettes, G. S. Bird, and J. W. Putney (2011)
Cold Spring Harb Perspect Biol 3, a004226
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Seeing is believing! Imaging Ca2+-signalling events in living cells.
J. G. McGeown (2010)
Exp Physiol 95, 1049-1060
   Abstract »    Full Text »    PDF »
Perspectives on: Local calcium signaling: Recording single-channel activity of inositol trisphosphate receptors in intact cells with a microscope, not a patch clamp.
I. Parker and I. F. Smith (2010)
J. Gen. Physiol. 136, 119-127
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