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Sci. Signal., 1 December 2009
Vol. 2, Issue 99, p. ra79
[DOI: 10.1126/scisignal.2000409]


Editor's Summary

Dual Nature of RIAM
Activation of T cells involves not only signaling downstream of the T cell receptor (TCR), but also activation of and signaling by integrins. The adaptor protein Rap1–guanosine triphosphate (GTP)–interacting adaptor molecule (RIAM) mediates inside-out activation of integrins in lymphocytes and participates in reorganization of the actin cytoskeleton. RIAM is also recruited to the interface between the T cell and an antigen-presenting cell; therefore, Patsoukis et al. investigated whether RIAM participated in the TCR signaling events that contribute to T cell activation. They found that RIAM bound to phospholipase C–{gamma}1 (PLC-{gamma}1) and, through interactions with the actin cytoskeleton, regulated the localization of PLC-{gamma}1, resulting in the production of inositol trisphophate (IP3) and diacylglycerol. Knockdown of RIAM in T cells resulted in defective activation of PLC-{gamma}1, diminished mobilization of Ca2+, and impaired translocation of the transcription factor NFAT to the nucleus in response to stimulation of the TCR. Thus, RIAM has multiple functions in T cell activation, regulating Ca2+ signaling and gene expression downstream of the TCR, integrin activity, and the actin cytoskeleton.

Citation: N. Patsoukis, E. M. Lafuente, P. Meraner, J. s. Kim, D. Dombkowski, L. Li, V. A. Boussiotis, RIAM Regulates the Cytoskeletal Distribution and Activation of PLC-{gamma}1 in T Cells. Sci. Signal. 2, ra79 (2009).

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