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Sci. Signal., 4 January 2011
Vol. 4, Issue 154, p. rs1
Coping with Damage
Genomic integrity is critical for cell and organismal survival; therefore, elaborate mechanisms have evolved to cope with DNA damage. By knocking down each Drosophila gene, Kondo and Perrimon identified 64 that were specifically important for the G2-M checkpoint and allowed continued mitosis after DNA damage with a drug that causes double-stranded DNA breaks. The authors verified the role of two complexes in the G2-M checkpoint through in vivo analysis in imaginal discs (clusters of cells that differentiate into adult tissues): the MUS312 nuclease complex that participates in resolving double-stranded breaks and the DNA replication preinitiation complex (pre-IC). The results of the screen also point to additional regulators of the G2-M checkpoint, thus paving the way for greater understanding of the differences and similarities between fly and vertebrate checkpoint responses.
Citation: S. Kondo, N. Perrimon, A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. Sci. Signal.4, rs1 (2011).