Sci. Signal., 15 March 2011
Inducible Regulation with ABASeveral systems exist to inducibly alter cellular behavior; however, they each have limitations. Turning to plants, Liang et al. determined that they could engineer systems with two protein components of the plant abscisic acid (ABA) pathway to create a chemically induced proximity system that responded to exogenously applied ABA (see the associated Perspective by Cutler). They demonstrated the ability of this ABA-induced proximity system to stimulate gene expression and control protein subcellular localization and activation of a signaling cascade in mammalian cells; furthermore, they showed that the ABA system could be combined with other induced proximity systems to control multiple proteins simultaneously. Studies with mice suggested that ABA was orally available and had a half-life suitable for analysis of biologic pathways. These characteristics of ABA, combined with its lack of toxicity, may make this system useful for applications in humans.
Citation: F.-S. Liang, W. Q. Ho, G. R. Crabtree, Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity. Sci. Signal. 4, rs2 (2011).
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