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Sci. Signal., 4 October 2011
Vol. 4, Issue 193, p. ra66
Focused on the Center
After T cell receptor (TCR) stimulation by an antigen-presenting cell (APC), an array of proteins are recruited to the T cell side of the interface with the APC (the immunological synapse), where they generate signals that activate the T cell. Singleton et al. provide data showing that interleukin-2 (IL-2)–inducible T cell kinase (Itk) plays a central role in directing "molecular traffic" at the immunological synapse. By comparing the organization of fluorescent sensors of T cell signaling molecules between antigen-activated wild-type and Itk-deficient T cells, the authors found that loss of Itk resulted in the inappropriate spatial organization of many signaling proteins at the T cell–APC interface, which impaired T cell activation. In particular, Itk was required to direct the activation of the guanosine triphosphatase Cdc42 at the center of the synapse, which led to appropriate actin accumulation downstream of TCR activation. Together, these data establish a central role for Itk in the organization of T cell signaling molecules and provide evidence for the dependence of critical T cell functions on the spatiotemporal organization of signaling intermediates.
Citation: K. L. Singleton, M. Gosh, R. D. Dandekar, B. B. Au-Yeung, O. Ksionda, V. L. J. Tybulewicz, A. Altman, D. J. Fowell, C. Wülfing, Itk Controls the Spatiotemporal Organization of T Cell Activation. Sci. Signal.4, ra66 (2011).
Ernesto Andrianantoandro and John F. Foley (31 July 2012) Sci. Signal.5 (235), eg8.
[DOI: 10.1126/scisignal.2003412] |Abstract »|Full Text »|PDF »
Leo M. Carlin, Rachel Evans, Hanna Milewicz, Luis Fernandes, Daniel R. Matthews, Michela Perani, James Levitt, Melanie D. Keppler, James Monypenny, Ton Coolen, Paul R. Barber, Borivoj Vojnovic, Klaus Suhling, Franca Fraternali, Simon Ameer-Beg, Peter J. Parker, N. Shaun B. Thomas, and Tony Ng (29 November 2011) Sci. Signal.4 (201), ra81.
[DOI: 10.1126/scisignal.2001729] |Editor's Summary »|Abstract »|Full Text »|PDF »|Supplementary Materials »
Mechanism and function of Vav1 localisation in TCR signalling.
O. Ksionda, A. Saveliev, R. Kochl, J. Rapley, M. Faroudi, J. E. Smith-Garvin, C. Wulfing, K. Rittinger, T. Carter, and V. L. J. Tybulewicz (2012)
J. Cell Sci.
|Abstract »|Full Text »|PDF »
DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.