Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Sci. Signal., 26 February 2013
Vol. 6, Issue 264, p. rs5
Complexes Reveal Signaling Dynamics
Analysis of high-throughput data sets can provide information about changes in gene expression, protein abundance, and signaling pathway activity. However, current data mining approaches do not identify changes to functional protein complexes within a pathway over time, a critical aspect for network analysis. Vinayagam et al. developed an interactive Web tool called COMPLEAT, which uses raw genome-wide, RNA interference data to map protein complex dynamics during the cellular response to stimuli in humans, flies, and yeast. Using phosphorylated extracellular signal–regulated kinase as a marker for pathway activation, COMPLEAT identified the Brahma complex in the cellular response to insulin, a prediction that was validated in a Drosophila cell line.
Citation: A. Vinayagam, Y. Hu, M. Kulkarni, C. Roesel, R. Sopko, S. E. Mohr, N. Perrimon, Protein Complex–Based Analysis Framework for High-Throughput Data Sets. Sci. Signal.6, rs5 (2013).
Ralph A. Neumüller, Thomas Gross, Anastasia A. Samsonova, Arunachalam Vinayagam, Michael Buckner, Karen Founk, Yanhui Hu, Sara Sharifpoor, Adam P. Rosebrock, Brenda Andrews, Fred Winston, and Norbert Perrimon (20 August 2013) Sci. Signal.6 (289), ra70.
[DOI: 10.1126/scisignal.2004145] |Editor's Summary »|Abstract »|Full Text »|PDF »|Supplementary Materials »
Adam A. Friedman, George Tucker, Rohit Singh, Dong Yan, Arunachalam Vinayagam, Yanhui Hu, Richard Binari, Pengyu Hong, Xiaoyun Sun, Maura Porto, Svetlana Pacifico, Thilakam Murali, Russell L. Finley, Jr., John M. Asara, Bonnie Berger, and Norbert Perrimon (25 October 2011) Sci. Signal.4 (196), rs10.
[DOI: 10.1126/scisignal.2002029] |Editor's Summary »|Abstract »|Full Text »|PDF »|Supplementary Materials »