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Open Forum on Methodology

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Antibodies to phosphorylated MAP kinase

8 November 2000

Eve De Lamirande

I recently bought antibodies (a mono- and a polyclonal) against activated MAP kinase (pMAPK). These antibodies were raised against doubly phosphorylated TEY (*TEY*) of the active center of MAPK. I observed that the antibodies recognize many proteins beside MAP kinase and that interesting things are happening with these other proteins at the time of activation/inhibition of signal transduction. This labelling does not appear unspecific as it changes with treatments and time course of cell activation.

Would anybody know of other proteins, beside MAP kinase, that have the *TEY* motif when they are activated?

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