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Open Forum on Methodology

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18 July 2003

Gavin J Clydesdale

Dear Forum Members

From a non-anonymous Gavin Clydesdale

I am having trouble establishing a reliable method to stimulate detectable levels of phos-c-Jun in Jurkat cells.

I am after a reliable method of stimulation that will cause normal (non-transfected)Jurkat cells to produce detectable amounts of phosphorylated c-Jun via western blotting ( and flow cytometry would also be helpful).

The details of the stimulating agent plus the optimal time point at which to assess the phosphorylation are important. References would also be helpful.

Thank you

Kind regards

Gavin Clydesdale (

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