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Open Forum on Methodology

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Response to antibodies to phospho MAPK

22 November 2000

Fabio Romerio

I have been using anti-phospho ERK1/2 antibodies for 2 years now. At first I used to get multiple bands as well but I have been able to get rid of them, leaving only the specific ERK1/2 bands. If the antibodies you are using are from Cell Signaling Technology (new England Biolabs spin-off company), then you may be able to improve your western blots by changing the conditions.

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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882