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Open Forum on Methodology

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Elution of GST fusion proteins

16 January 2001

Mary Harte

We have a GST fusion protein that we are not able to elute off of the glutathione agarose using 20mM glutathione in 50mM tris -HCl pH8. Does anyone have any suggestions for eluting this fusion protein from the beads? Thanks, Mary Harte

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