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Principles of Cell Signaling and Biological Consequences

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Group B: Discussion Question 1

31 January 2005

Andrew Chan

The MAPK kinase pathway is composed of several classes of signaling molecules, such as receptors, adaptors, G proteins, phosphatases, and various kinases. One commonly used research tool for unraveling the biological functions of signaling molecules is dominant-negative (DN) mutants. Select a signaling molecule in this pathway and (i) describe how you would construct a dominant-negative mutant, (ii) explain how it works as a DN mutant, and (iii) describe some of the pitfalls in using that particular mutant.

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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882