Principles of Cell Signaling and Biological Consequences
Group B: Discussion Question 1
31 January 2005
The MAPK kinase pathway is composed of several classes of signaling molecules, such as receptors, adaptors, G proteins, phosphatases, and various kinases. One commonly used research tool for unraveling the biological functions of signaling molecules is dominant-negative (DN) mutants. Select a signaling molecule in this pathway and (i) describe how you would construct a dominant-negative mutant, (ii) explain how it works as a DN mutant, and (iii) describe some of the pitfalls in using that particular mutant.
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882