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Open Forum on Cell Signaling

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What do you say about using 2-D proteomics?

16 September 2002

Dk Singh

Hello members, What do you think about the idea of using 2-D electrophoresis using the membrane fraction of the cells for topology mapping? I do feel that this will not only give one an outlook of the resting cells as such but also provide information about the changes in the cells' membrane status both in normal resting conditions and in activated states. Please comment on the feasibility of the process.

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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882