|
ST NetWatch: Technical Information
-
Cell Biology Laboratory Manual
- The Cell Biology Laboratory Manual, authored by William Heidcamp at Gustavus Adolphus College, was designed for use in cell biology laboratory courses. It is valuable as both a practical reference and a teaching tool because it covers a wide range of techniques and model systems used in cell and molecular biology research. The manual includes chapters on cell culture, the cytoskeleton, histochemistry, spectrophotometry, and photosynthesis and respiration. Basic methods such as gel electrophoresis are included as well as more advanced techniques such as cell fractionation and electron microscopy. There is a large collection of recipes and preparation instructions for many solutions and reagents in the Appendix, and a basic statistical analysis primer.
(Free Site)
-
DrugBank
- Do you want to know if a drug you are using in experiments affects only one pathway, or several? Do you want to find drugs that act on your favorite pathway? DrugBank is a database of drug-target interactions that researchers using pharmacological agents may find useful. Search the site by gene name or sequence to find drugs that affect a particular protein target, or search by drug name or structure to determine what pathways are affected by a particular drug. The database includes information for many FDA-approved and experimental drugs, biotech drugs, illicit substances and small molecule drugs. DrugBank is supported by Genome Alberta and Genome Canada in cooperation with GenomeQuest, Inc.
(Free Site)
-
Ki Database
- The Ki database is a searchable index of affinity information about drugs and the receptors, channels and enzymes they target. You can search the database by receptor, species, ligand, Ki, expressing tissue or any combination of these descriptors. References for the reported Kis are clearly indicated so that users can determine under what conditions they were obtained . The database was generated by Bryan Roth’s lab at UNC and includes information from published research as well as data from the National Institute of Mental Health (NIMH) Psychoactive Drug Screening Program. A selection of cDNA clones of receptors and transporters identified by this large-scale NIMH screen are available for academic use.
(Free Site)
-
NCBI Minicourses
- NCBI offers several mini-courses that teach users how to get the most out of NCBI’s bioinformatics tools such as BLAST, Greengene and Entrez Gene. Most of these courses are offered in the real world at various host institutions around the United States, but some have online components that could serve as stand-alone tutorials for novices or as a refresher for those who may not have used all of NCBI’s bioinformatics tools recently. There are BLAST, Entrez Gene and structural analysis QuickStart guides available. For casual users of bioinformatics tools, these mini-courses reveal the deeper capabilities of the applications and highlight just how much information a well-informed user can glean from bioinformatics. If you cannot attend a mini-course, check out the online components that are available. Make sure you are getting the most out of your sequence data by learning more about the software you use for analysis.
(Free Site)
-
Protocol Online
- These free protocols are contributed by academic research labs, biotechnology companies, and scientific community-based programs such as genome sequencing projects and model organism databases. Scroll past the advertisements that appear at the top of each page and you will find a variety of protocols relevant to many areas of cell biology, biochemistry, and molecular biology. The collection includes basic information, such as recipes for common molecular biology reagents, but also provides many protocols for advanced and specialized techniques. You can find protocols on topics that range from transforming lymphocytes, to culturing cerebellar neurons, to doing erythrophagocytosis assays, to performing surgical procedures such as xenografts. There are numerous yeast and C. elegans protocols listed under the “Model Organisms” category. Most protocols are provided in the usual style step-by-step written instructions, but several are provided as video tutorials, such as those describing laser-capture microdissection and hanging-drop culture of embryoid bodies. So if you would like to find out how to process histology samples in the microwave or isolate chloroplasts from spinach, take a look around this site.
(Free Site)
-
WormMethods
- WormMethods is part of the WormBook web site, produced by and for the C. elegans research community. This page contains standard methods used for genetics, neurophysiology, and cell biology in the worm, as well as some molecular biology methods that can be used with any system. Worm-specific protocols include design of genetic screens, RNA in situ analysis, and husbandry. Protocols for procedures such as yeast two-hybrid and microarray screening are readily adaptable to other systems and thus would be useful to molecular and cell biologists outside the worm community.
(Free Site)
|
|
