Editors' ChoiceCell Biology

Mapping the Yeast Protein Interaction Network

Science Signaling  17 Jun 2008:
Vol. 1, Issue 24, pp. ec226
DOI: 10.1126/scisignal.124ec226

To move beyond genes to an understanding of networks, it is necessary to track protein-protein interactions in vivo. Tarassov et al. have used protein-fragment complementation assays, which are based on reassembly of two domains of the enzyme dihydrofolate reductase that have been fused to the proteins of interest, to look at the protein interaction network, the interactome, in yeast. In addition to confirming known interactions within complexes, insights were obtained into the network underlying autophagy, a conserved process by which cells digest their own constituents in response to starvation, and a network underlying cellular polarization during the cell cycle.

K. Tarassov, V. Messier, C. R. Landry, S. Radinovic, M. M. Serna Molina, I. Shames, Y. Malitskaya, J. Vogel, H. Bussey, S. W. Michnick, An in vivo map of the yeast protein interactome. Science 320 (5882), 1465-1470 (2008). [Abstract] [Full Text]